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The Faculty of Medicine at Wasit University discusses a Master’s thesis on the molecular and immunological diagnosis of opportunistic intestinal protozoa in haemodialysis patients
The Faculty of Medicine at Wasit University examined the Master’s thesis by student Doaa Amer Shamkhi, entitled ‘A Molecular and Immunological Diagnostic Study of Opportunistic Intestinal Protozoan Infections in Haemodialysis Patients in Wasit Governorate’.
The thesis aimed to detect infection with opportunistic intestinal protozoa in haemodialysis patients, to compare the efficacy of microscopic diagnosis with the nested polymerase chain reaction (PCR) technique, and to assess the levels of a number of immunological markers, including interleukin-5 (IL-5) and interleukin-6 (IL-6).
The study involved molecular analysis and genetic characterisation of a number of intestinal parasites, resulting in the identification of nine genetic sequences from Iraqi isolates, comprising three sequences each for (Cryptosporidium parvum), (Blastocystis hominis) and (Entamoeba coli), which were deposited in the National Centre for Biotechnology Information (NCBI GenBank) database, thereby enhancing the genetic database for these parasites and opening up prospects for their use in future research studies.
The examination committee consisted of Professor Ghada Basil Ali as chair, with Professor Sabah Fadel Al-Quraishi, Assistant Professor Ghusq Jabr Shamkhi and Professor Baraa Abdulsalam Harija serving as members and supervisors.
Following an in-depth discussion of the thesis and the scientific findings presented by the researcher, the committee awarded the student a ‘Distinction’ grade, commending the importance of the study’s topic, the scientific effort expended, and the results presented, which contribute to advancing scientific knowledge regarding the molecular and immunological diagnosis of intestinal parasitic infections in haemodialysis patients.
This defence forms part of the University’s commitment to

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